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TitleGenetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics.
Journal, issue, pagesFront Mol Neurosci, Vol. 6, Page 2-2, Year 2013
Publish dateSep 28, 2011 (structure data deposition date)
AuthorsAkerboom, J. / Carreras Calderon, N. / Tian, L. / Wabnig, S. / Prigge, M. / Tolo, J. / Gordus, A. / Orger, M.B. / Severi, K.E. / Macklin, J.J. ...Akerboom, J. / Carreras Calderon, N. / Tian, L. / Wabnig, S. / Prigge, M. / Tolo, J. / Gordus, A. / Orger, M.B. / Severi, K.E. / Macklin, J.J. / Patel, R. / Pulver, S.R. / Wardill, T.J. / Fischer, E. / Schuler, C. / Chen, T.W. / Sarkisyan, K.S. / Marvin, J.S. / Bargmann, C.I. / Kim, D.S. / Kugler, S. / Lagnado, L. / Hegemann, P. / Gottschalk, A. / Schreiter, E.R. / Looger, L.L.
External linksFront Mol Neurosci / PubMed:23459413
MethodsX-ray diffraction
Resolution1.25 - 2.2 Å
Structure data

PDB-3u0k:
Crystal Structure of the genetically encoded calcium indicator RCaMP
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-3u0l:
Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 4.5
Method: X-RAY DIFFRACTION / Resolution: 1.25 Å

PDB-3u0m:
Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 8.5
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-3u0n:
Crystal structure of the engineered fluorescent protein mRuby, crystal form 2
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-4i2y:
Crystal Structure of the genetically encoded calcium indicator RGECO1
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-ACT:
ACETATE ION

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

Source
  • entacmaea quadricolor (sea anemone)
  • gallus gallus (chicken)
  • discosoma sp. (sea anemone)
  • rattus norvegicus (Norway rat)
KeywordsFLUORESCENT PROTEIN / calcium binding / EF-hand / genetically encoded calcium indicator / sensor / mApple / fluorescent calcium indicator

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