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TitleCryo-EM structures of Arx1 and maturation factors Rei1 and Jjj1 bound to the 60S ribosomal subunit.
Journal, issue, pagesNat Struct Mol Biol, Vol. 19, Issue 12, Page 1228-1233, Year 2012
Publish dateNov 11, 2012
AuthorsBasil J Greber / Daniel Boehringer / Christian Montellese / Nenad Ban /
PubMed AbstractEukaryotic ribosome biogenesis requires many protein factors that facilitate the assembly, nuclear export and final maturation of 40S and 60S particles. We have biochemically characterized ribosomal ...Eukaryotic ribosome biogenesis requires many protein factors that facilitate the assembly, nuclear export and final maturation of 40S and 60S particles. We have biochemically characterized ribosomal complexes of the yeast 60S-biogenesis factor Arx1 and late-maturation factors Rei1 and Jjj1 and determined their cryo-EM structures. Arx1 was visualized bound to the 60S subunit together with Rei1, at 8.1-Å resolution, to reveal the molecular details of Arx1 binding whereby Arx1 arrests the eukaryotic-specific rRNA expansion segment 27 near the polypeptide tunnel exit. Rei1 and Jjj1, which have been implicated in Arx1 recycling, bind in the vicinity of Arx1 and form a network of interactions. We suggest that, in addition to the role of Arx1 during pre-60S nuclear export, the binding of Arx1 conformationally locks the pre-60S subunit and inhibits the premature association of nascent chain-processing factors to the polypeptide tunnel exit.
External linksNat Struct Mol Biol / PubMed:23142985
MethodsEM (single particle)
Resolution8.1 - 16.3 Å
Structure data

EMDB-2167:
Cryo-EM structure of the 60S-Arx1-Rei1-Jjj1 complex
Method: EM (single particle) / Resolution: 16.3 Å

EMDB-2168:
Cryo-EM structure of the 60S-Rei1 complex
Method: EM (single particle) / Resolution: 14.6 Å

2169

4v8t

EMDB-2169: Cryo-EM structure of the 60S-Arx1-Rei1 complex
PDB-4v8t: Cryo-EM Structure of the 60S Ribosomal Subunit in Complex with Arx1 and Rei1
Method: EM (single particle) / Resolution: 8.1 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsRIBOSOME / LARGE RIBOSOMAL SUBUNIT / RIBOSOME BIOGENESIS / RIBOSOME MATURATION FACTOR

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