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TitleApplying pairwise combinations of amino Acid mutations for sorting out highly efficient glucosylation tools for chemo-enzymatic synthesis of bacterial oligosaccharides.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 134, Page 18677-18688, Year 2012
Publish dateJun 15, 2012 (structure data deposition date)
AuthorsChampion, E. / Guerin, F. / Moulis, C. / Barbe, S. / Tran, T.H. / Morel, S. / Descroix, K. / Monsan, P. / Mourey, L. / Mulard, L.A. ...Champion, E. / Guerin, F. / Moulis, C. / Barbe, S. / Tran, T.H. / Morel, S. / Descroix, K. / Monsan, P. / Mourey, L. / Mulard, L.A. / Tranier, S. / Remaud-Simeon, M. / Andre, I.
External linksJ. Am. Chem. Soc. / PubMed:23072374
MethodsX-ray diffraction
Resolution2.2 - 2.5 Å
Structure data

PDB-4flo:
Crystal structure of Amylosucrase double mutant A289P-F290C from Neisseria polysaccharea
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-4flq:
Crystal structure of Amylosucrase double mutant A289P-F290I from Neisseria polysaccharea.
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-4flr:
Crystal structure of Amylosucrase double mutant A289P-F290L from Neisseria polysaccharea
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-4fls:
Crystal structure of Amylosucrase inactive double mutant F290K-E328Q from Neisseria polysaccharea in complex with sucrose.
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-GOL:
GLYCEROL

ChemComp-P6G:
HEXAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-CL:
Unknown entry

Source
  • neisseria polysaccharea (bacteria)
KeywordsTRANSFERASE / BETA/ALPHA-BARREL / GLYCOSIDE HYDROLASE / AMYLOSE SYNTHESIS / SUCROSE ISOMERIZATION / GLUCOSYLTRANSFERASE / CARBOHYDRATE / GLUCOSYLTRANSFERASE CARBOHYDRATE

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