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-Structure paper
Title | The tetramer of p53 in the absence of DNA forms a relaxed quaternary state. |
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Journal, issue, pages | Biochemistry, Vol. 51, Issue 41, Page 8053-8055, Year 2012 |
Publish date | Oct 16, 2012 |
Authors | Nam Pham / Armando Lucumi / Nikki Cheung / Hector Viadiu / |
PubMed Abstract | p53 is a tetrameric multidomain protein that triggers the anticancer cellular response to stress. We have calculated a three-dimensional reconstruction of full-length human p53 in the absence of DNA ...p53 is a tetrameric multidomain protein that triggers the anticancer cellular response to stress. We have calculated a three-dimensional reconstruction of full-length human p53 in the absence of DNA using single-particle electron microscopy. The reconstruction of DNA-free full-length p53 shows a square-shaped structure with four distinct domains and a hollow center. In comparison with the known compacted DNA-bound full-length p53 structures, the DNA-free p53 tetramer adopts a relaxed conformation with separated monomers and oligomerization interfaces different from those of the DNA-bound conformation. |
External links | Biochemistry / PubMed:23025236 |
Methods | EM (single particle) |
Structure data | EMDB-5342: |
Source |
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