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Structure paper

TitleSingle Amino Acid Exchange in Bacteriophage Hk620 Tailspike Protein Results in Thousand-Fold Increase of its Oligosaccharide Affinity.
Journal, issue, pagesGlycobiology, Vol. 23, Page 59-, Year 2013
Publish dateFeb 22, 2010 (structure data deposition date)
AuthorsBroeker, N.K. / Gohlke, U. / Muller, J.J. / Uetrecht, C. / Heinemann, U. / Seckler, R. / Barbirz, S.
External linksGlycobiology / PubMed:22923442
MethodsX-ray diffraction
Resolution1.35 - 1.82 Å
Structure data

PDB-2x6w:
Tailspike protein mutant E372Q of E.coli bacteriophage HK620 in complex with hexasaccharide
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-2x6x:
Tailspike protein mutant D339N of E.coli bacteriophage HK620 in complex with hexasaccharide
Method: X-RAY DIFFRACTION / Resolution: 1.48 Å

PDB-2x6y:
Tailspike protein mutant D339A of E.coli bacteriophage HK620 in complex with hexasaccharide
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-2x85:
Tailspike protein of E. coli bacteriophage HK620 in complex with hexasaccharide
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-4avz:
Tailspike protein mutant E372Q of E. coli bacteriophage HK620
Method: X-RAY DIFFRACTION / Resolution: 1.82 Å

Chemicals

ChemComp-NA:
Unknown entry

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-HOH:
WATER

ChemComp-144:
TRIS-HYDROXYMETHYL-METHYL-AMMONIUM

Source
  • enterobacteria phage hk620 (virus)
  • salmonella phage hk620 (virus)
KeywordsVIRAL PROTEIN / BETA-HELIX / HYDROLASE / RIGHT-HANDED PARALLEL BETA-HELIX / VIRAL ADHESION PROTEIN / ENDO-N-ACETYLGLUCOSAMINIDASE / TAILSPIKE / O-ANTIGEN

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