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TitleDevelopment of a tetrameric streptavidin mutein with reversible biotin binding capability: engineering a mobile loop as an exit door for biotin.
Journal, issue, pagesPlos One, Vol. 7, Page e35203-e35203, Year 2012
Publish dateApr 9, 2012 (structure data deposition date)
AuthorsO'Sullivan, V.J. / Barrette-Ng, I. / Hommema, E. / Hermanson, G.T. / Schofield, M. / Wu, S.C. / Honetschlaeger, C. / Ng, K.K. / Wong, S.L.
External linksPlos One / PubMed:22536357
MethodsX-ray diffraction
Resolution2 Å
Structure data

PDB-4ekv:
Streptavidin 8-aa-loop H127C mutein with reversible biotin binding
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-BTN:
BIOTIN

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

Source
  • streptomyces avidinii (bacteria)
Keywordsbiotin-binding protein / Beta-barrel / Binding protein / Biotin-binding

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