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Structure paper

TitleNon-Specific Protein-DNA Interactions Control I-Crei Target Binding and Cleavage.
Journal, issue, pagesNucleic Acids Res., Vol. 40, Page 6936-6945, Year 2012
Publish dateDec 1, 2011 (structure data deposition date)
AuthorsMolina, R. / Redondo, P. / Stella, S. / Marenchino, M. / D'Abramo, M. / Gervasio, F.L. / Charles Epinat, J. / Valton, J. / Grizot, S. / Duchateau, P. ...Molina, R. / Redondo, P. / Stella, S. / Marenchino, M. / D'Abramo, M. / Gervasio, F.L. / Charles Epinat, J. / Valton, J. / Grizot, S. / Duchateau, P. / Prieto, J. / Montoya, G.
External linksNucleic Acids Res. / PubMed:22495931
MethodsX-ray diffraction
Resolution2.5 - 3.1 Å
Structure data

PDB-4aab:
Crystal structure of the mutant D75N I-CreI in complex with its wild- type target (The four central bases, 2NN region, are composed by GTAC from 5' to 3')
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-4aad:
Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in absence of metal ions at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3')
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

PDB-4aae:
Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by AGCG from 5' to 3')
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-4aaf:
Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3')
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-4aag:
Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in presence of Ca at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3')
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

Chemicals

ChemComp-PGO:
S-1,2-PROPANEDIOL

ChemComp-MG:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-CA:
Unknown entry

Source
  • chlamydomonas reinhardtii (plant)
  • synthetic construct (others)
KeywordsHYDROLASE/DNA / HYDROLASE-DNA COMPLEX / GENE TARGETING / PROTEIN-DNA INTERACTION / HOMING ENDONUCLEASES

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