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Structure paper

TitleA structural basis for reversible photoswitching of absorbance spectra in red fluorescent protein rsTagRFP.
Journal, issue, pagesJ. Mol. Biol., Vol. 417, Page 144-151, Year 2012
Publish dateOct 16, 2011 (structure data deposition date)
AuthorsPletnev, S. / Subach, F.V. / Dauter, Z. / Wlodawer, A. / Verkhusha, V.V.
External linksJ. Mol. Biol. / PubMed:22310052
MethodsX-ray diffraction
Resolution1.783 - 2.188 Å
Structure data

PDB-3u8a:
Crystal structure of monomeric reversibly photoswitchable red fluorescent protein rsTagRFP in the OFF state
Method: X-RAY DIFFRACTION / Resolution: 1.783 Å

PDB-3u8c:
Crystal structure of monomeric reversibly photoswitchable red fluorescent protein rsTagRFP in the ON state
Method: X-RAY DIFFRACTION / Resolution: 2.188 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

Source
  • synthetic construct (others)
KeywordsFLUORESCENT PROTEIN / beta-barrel / light induced reversible photoswitching / reversibly photoswitchable fluorescent protein / cis-trans isomerization / beta barrel

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