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TitleStructure and operation of the DNA-translocating type I DNA restriction enzymes.
Journal, issue, pagesGenes Dev, Vol. 26, Issue 1, Page 92-9104, Year 2012
Publish dateJan 1, 2012
AuthorsChristopher K Kennaway / James E N Taylor / Chun Feng Song / Wojciech Potrzebowski / William Nicholson / John H White / Anna Swiderska / Agnieszka Obarska-Kosinska / Philip Callow / Laurie P Cooper / Gareth A Roberts / Jean-Baptiste Artero / Janusz M Bujnicki / John Trinick / G Geoff Kneale / David T F Dryden /
PubMed AbstractType I DNA restriction/modification (RM) enzymes are molecular machines found in the majority of bacterial species. Their early discovery paved the way for the development of genetic engineering. ...Type I DNA restriction/modification (RM) enzymes are molecular machines found in the majority of bacterial species. Their early discovery paved the way for the development of genetic engineering. They control (restrict) the influx of foreign DNA via horizontal gene transfer into the bacterium while maintaining sequence-specific methylation (modification) of host DNA. The endonuclease reaction of these enzymes on unmethylated DNA is preceded by bidirectional translocation of thousands of base pairs of DNA toward the enzyme. We present the structures of two type I RM enzymes, EcoKI and EcoR124I, derived using electron microscopy (EM), small-angle scattering (neutron and X-ray), and detailed molecular modeling. DNA binding triggers a large contraction of the open form of the enzyme to a compact form. The path followed by DNA through the complexes is revealed by using a DNA mimic anti-restriction protein. The structures reveal an evolutionary link between type I RM enzymes and type II RM enzymes.
External linksGenes Dev / PubMed:22215814 / PubMed Central
MethodsEM (single particle)
Resolution20.0 - 35.0 Å
Structure data

EMDB-1890:
EcoR124 Type I DNA restriction-modification enzyme complex in closed state with bound 30bp cognate DNA fragment. 3D reconstruction by single particle analysis from negative stain EM.
Method: EM (single particle) / Resolution: 21.0 Å

EMDB-1891:
EcoR124 Type I DNA restriction-modification enzyme complex without DNA (open state). Low resolution 3D reconstruction by single particle analysis from negative stain EM.
Method: EM (single particle) / Resolution: 35.0 Å

EMDB-1892:
EcoR124 Type I DNA restriction-modification enzyme complex (in closed state) with bound DNA mimic protein Ocr from phage T7. 3D reconstruction by single particle analysis from negative stain EM.
Method: EM (single particle) / Resolution: 24.0 Å

EMDB-1893:
EcoKI Type I DNA restriction-modification enzyme complex in closed state with bound 75bp cognate DNA fragment. 3D reconstruction by single particle analysis from negative stain EM.
Method: EM (single particle) / Resolution: 20.0 Å

Source
  • Escherichia coli (E. coli)
  • synthetic construct (others)
  • Enterobacteria phage T7 (virus)

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