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TitleThree-dimensional structure of basal body triplet revealed by electron cryo-tomography.
Journal, issue, pagesEMBO J, Vol. 31, Issue 3, Page 552-562, Year 2012
Publish dateFeb 1, 2012
AuthorsSam Li / Jose-Jesus Fernandez / Wallace F Marshall / David A Agard /
PubMed AbstractBasal bodies and centrioles play central roles in microtubule (MT)-organizing centres within many eukaryotes. They share a barrel-shaped cylindrical structure composed of nine MT triplet blades. ...Basal bodies and centrioles play central roles in microtubule (MT)-organizing centres within many eukaryotes. They share a barrel-shaped cylindrical structure composed of nine MT triplet blades. Here, we report the structure of the basal body triplet at 33 Å resolution obtained by electron cryo-tomography and 3D subtomogram averaging. By fitting the atomic structure of tubulin into the EM density, we built a pseudo-atomic model of the tubulin protofilaments at the core of the triplet. The 3D density map reveals additional densities that represent non-tubulin proteins attached to the triplet, including a large inner circular structure in the basal body lumen, which functions as a scaffold to stabilize the entire basal body barrel. We found clear longitudinal structural variations along the basal body, suggesting a sequential and coordinated assembly mechanism. We propose a model in which δ-tubulin and other components participate in the assembly of the basal body.
External linksEMBO J / PubMed:22157822 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution33.0 Å
Structure data

EMDB-5252:
electron cryo-tomography reconstruction and subvolume averaging of the basal body triplet from Chlamydomonas reinhardtii
Method: EM (subtomogram averaging) / Resolution: 33.0 Å

Source
  • Chlamydomonas reinhardtii (plant)

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