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TitleStructure and dynamics of the mammalian ribosomal pretranslocation complex.
Journal, issue, pagesMol Cell, Vol. 44, Issue 2, Page 214-224, Year 2011
Publish dateOct 21, 2011
AuthorsTatyana Budkevich / Jan Giesebrecht / Roger B Altman / James B Munro / Thorsten Mielke / Knud H Nierhaus / Scott C Blanchard / Christian M T Spahn /
PubMed AbstractAlthough the structural core of the ribosome is conserved in all kingdoms of life, eukaryotic ribosomes are significantly larger and more complex than their bacterial counterparts. The extent to ...Although the structural core of the ribosome is conserved in all kingdoms of life, eukaryotic ribosomes are significantly larger and more complex than their bacterial counterparts. The extent to which these differences influence the molecular mechanism of translation remains elusive. Multiparticle cryo-electron microscopy and single-molecule FRET investigations of the mammalian pretranslocation complex reveal spontaneous, large-scale conformational changes, including an intersubunit rotation of the ribosomal subunits. Through structurally related processes, tRNA substrates oscillate between classical and at least two distinct hybrid configurations facilitated by localized changes in their L-shaped fold. Hybrid states are favored within the mammalian complex. However, classical tRNA positions can be restored by tRNA binding to the E site or by the eukaryotic-specific antibiotic and translocation inhibitor cycloheximide. These findings reveal critical distinctions in the structural and energetic features of bacterial and mammalian ribosomes, providing a mechanistic basis for divergent translation regulation strategies and species-specific antibiotic action.
External linksMol Cell / PubMed:22017870 / PubMed Central
MethodsEM (single particle)
Resolution9.0 - 10.6 Å
Structure data

5326

3j0l

EMDB-5326: Cryo EM reconstruction of mammalian 80S ribosome in classic PRE state 1
PDB-3j0l: Core of mammalian 80S pre-ribosome in complex with tRNAs fitted to a 9.8A cryo-EM map: classic PRE state 1
Method: EM (single particle) / Resolution: 9.8 Å

5327

3j0o

EMDB-5327: Cryo EM reconstruction of mammalian 80S ribosome in classic PRE state 2
PDB-3j0o: Core of mammalian 80S pre-ribosome in complex with tRNAs fitted to a 9A cryo-EM map: classic PRE state 2
Method: EM (single particle) / Resolution: 9.0 Å

5328

3j0p

EMDB-5328: Cryo EM reconstruction of mammalian 80S ribosome in rotated PRE state 1
PDB-3j0p: Core of mammalian 80S pre-ribosome in complex with tRNAs fitted to a 10.6A cryo-em map: rotated PRE state 1
Method: EM (single particle) / Resolution: 10.6 Å

5329

3j0q

EMDB-5329: Cryo EM reconstruction of mammalian 80S ribosome in rotated PRE state 2
PDB-3j0q: Core of mammalian 80S pre-ribosome in complex with tRNAs fitted to a 10.6A cryo-em map: rotated PRE state 2
Method: EM (single particle) / Resolution: 10.6 Å

Source
  • oryctolagus cuniculus (rabbit)
KeywordsRIBOSOME / Mammalia / translation / elongation cycle / tRNA

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