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TitleComplete set of glycosyltransferase structures in the calicheamicin biosynthetic pathway reveals the origin of regiospecificity.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 108, Page 17649-17654, Year 2011
Publish dateJul 13, 2009 (structure data deposition date)
AuthorsChang, A. / Singh, S. / Helmich, K.E. / Goff, R.D. / Bingman, C.A. / Thorson, J.S. / Phillips, G.N.
External linksProc. Natl. Acad. Sci. USA / PubMed:21987796
MethodsX-ray diffraction
Resolution1.597 - 2.505 Å
Structure data

PDB-3ia7:
Crystal Structure of CalG4, the Calicheamicin Glycosyltransferase
Method: X-RAY DIFFRACTION / Resolution: 1.91 Å

PDB-3iaa:
Crystal Structure of CalG2, Calicheamicin Glycosyltransferase, TDP bound form
Method: X-RAY DIFFRACTION / Resolution: 2.505 Å

PDB-3otg:
Crystal Structure of CalG1, Calicheamicin Glycostyltransferase, TDP bound form
Method: X-RAY DIFFRACTION / Resolution: 2.08 Å

PDB-3oth:
Crystal Structure of CalG1, Calicheamicin Glycostyltransferase, TDP and calicheamicin alpha3I bound form
Method: X-RAY DIFFRACTION / Resolution: 2.301 Å

PDB-3oti:
Crystal Structure of CalG3, Calicheamicin Glycostyltransferase, TDP and calicheamicin T0 bound form
Method: X-RAY DIFFRACTION / Resolution: 1.597 Å

PDB-3rsc:
Crystal Structure of CalG2, Calicheamicin Glycosyltransferase, TDP and calicheamicin T0 bound form
Method: X-RAY DIFFRACTION / Resolution: 2.19 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-TYD:
THYMIDINE-5'-DIPHOSPHATE

ChemComp-CLJ:
Calicheamicin alpha3I

ChemComp-C0T:
Calicheamicin T0

ChemComp-PO4:
PHOSPHATE ION

Source
  • micromonospora echinospora (bacteria)
KeywordsTRANSFERASE / Glycosysltransferase / Calicheamicin / CalG4 / enediyne / Glycosyltransferase / CalG2 / TDP / Structural Genomics / PSI-2 / Protein Structure Initiative / Center for Eukaryotic Structural Genomics / CESG / GT-B fold / Enzyme Discovery for Natural Product Biosynthesis / NatPro / transferase/antibiotic / transferase-antibiotic complex

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