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TitleSelf-directed assembly and clustering of the cytoplasmic domains of inwardly rectifying Kir2.1 potassium channels on association with PSD-95.
Journal, issue, pagesBiochim Biophys Acta, Vol. 1808, Issue 10, Page 2374-2389, Year 2011
Publish dateJul 5, 2011
AuthorsSvetlana Fomina / Tina D Howard / Olivia K Sleator / Marina Golovanova / Liam O'Ryan / Mark L Leyland / J Günter Grossmann / Richard F Collins / Stephen M Prince /
PubMed AbstractThe interaction of the extra-membranous domain of tetrameric inwardly rectifying Kir2.1 ion channels (Kir2.1NC(4)) with the membrane associated guanylate kinase protein PSD-95 has been studied using ...The interaction of the extra-membranous domain of tetrameric inwardly rectifying Kir2.1 ion channels (Kir2.1NC(4)) with the membrane associated guanylate kinase protein PSD-95 has been studied using Transmission Electron Microscopy in negative stain. Three types of complexes were observed in electron micrographs corresponding to a 1:1 complex, a large self-enclosed tetrad complex and extended chains of linked channel domains. Using models derived from small angle X-ray scattering experiments in which high resolution structures from X-ray crystallographic and Nuclear Magnetic Resonance studies are positioned, the envelopes from single particle analysis can be resolved as a Kir2.1NC(4):PSD-95 complex and a tetrad of this unit (Kir2.1NC(4):PSD-95)(4). The tetrad complex shows the close association of the Kir2.1 cytoplasmic domains and the influence of PSD-95 mediated self-assembly on the clustering of these channels.
External linksBiochim Biophys Acta / PubMed:21756874
MethodsEM (single particle) / small angle scattering
Resolution17.2 - 25.8 Å
Structure data

1761

2xkx

EMDB-1761, PDB-2xkx:
Single particle analysis of PSD-95 in negative stain
Method: EM (single particle) / Resolution: 22.9 Å

1764

2xky

EMDB-1764, PDB-2xky:
Single particle analysis of Kir2.1NC_4 in negative stain
Method: EM (single particle) / Resolution: 17.2 Å

EMDB-1765:
Single particle analysis of the 1:1 complex of PSD-95 and Kir2.1NC_4 in negative stain
Method: EM (single particle) / Resolution: 20.5 Å

EMDB-1766:
Single particle analysis of a tetramer of a subunit comprising the 1:1 complex of PSD-95 and Kir2.1NC_4, in negative stain
Method: EM (single particle) / Resolution: 25.8 Å

Source
  • rattus norvegicus (Norway rat)
  • mus musculus (house mouse)
KeywordsSTRUCTURAL PROTEIN / SCAFFOLD PROTEIN / MEMBRANE ASSOCIATED GUANYLATE KINASE / METAL TRANSPORT / ION CHANNEL / MEMBRANE PROTEIN

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