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TitleStructural comparison of HIV-1 envelope spikes with and without the V1/V2 loop.
Journal, issue, pagesJ Virol, Vol. 85, Issue 6, Page 2741-2750, Year 2011
Publish dateDec 29, 2010
AuthorsGuiqing Hu / Jun Liu / Kenneth A Taylor / Kenneth H Roux /
PubMed AbstractWe have used cryoelectron tomography of vitreous-ice-embedded HIV-1 virions to compare the envelope (Env) spikes of a wild-type strain with those of a mutant strain in which the V1/V2 loop has been ...We have used cryoelectron tomography of vitreous-ice-embedded HIV-1 virions to compare the envelope (Env) spikes of a wild-type strain with those of a mutant strain in which the V1/V2 loop has been deleted. Deletion of V1/V2 results in a spike with far more structural heterogeneity than is observed in the wild type, likely reflecting greatly enhanced gp120 protomer flexibility. A major difference between the two forms is a pronounced loss of mass from the "peak" of the native Env spike. The apparent loss of contact among three gp120 protomers likely accounts for the more open structure, heterogeneity in configuration, and previous observations that broadly neutralizing epitopes and reactive sites on other structural elements are more exposed in such constructs.
External linksJ Virol / PubMed:21191026 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution33.0 Å
Structure data

EMDB-1813:
Structural Comparison of HIV-1 Envelope Spikes with and without the V1V2 Loop.
Method: EM (subtomogram averaging) / Resolution: 33.0 Å

EMDB-1814:
Structural Comparison of HIV-1 Envelope Spikes with and without the V1V2 Loop
Method: EM (subtomogram averaging) / Resolution: 33.0 Å

Source
  • Human immunodeficiency virus 1

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