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-Structure paper
タイトル | Cryo-EM analysis reveals new insights into the mechanism of action of pyruvate carboxylase. |
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ジャーナル・号・ページ | Structure, Vol. 18, Issue 10, Page 1300-1310, Year 2010 |
掲載日 | 2010年10月13日 |
著者 | Gorka Lasso / Linda P C Yu / David Gil / Song Xiang / Liang Tong / Mikel Valle / |
PubMed 要旨 | Pyruvate carboxylase (PC) is a conserved multifunctional enzyme linked to important metabolic diseases. PC homotetramer is arranged in two layers with two opposing monomers per layer. Cryo-EM ...Pyruvate carboxylase (PC) is a conserved multifunctional enzyme linked to important metabolic diseases. PC homotetramer is arranged in two layers with two opposing monomers per layer. Cryo-EM explores the conformational variability of PC in the presence of different substrates. The results demonstrate that the biotin-carboxyl carrier protein (BCCP) domain localizes near the biotin carboxylase (BC) domain of its own monomer and travels to the carboxyltransferase (CT) domain of the opposite monomer. All density maps show noticeable conformational differences between layers, mainly for the BCCP and BC domains. This asymmetry may be indicative of a coordination mechanism where monomers from different layers catalyze the BC and CT reactions consecutively. A conformational change of the PC tetramerization (PT) domain suggests a new functional role in communication. A long-range communication pathway between subunits in different layers, via interacting PT-PT and BC-BC domains, may be responsible for the cooperativity of PC from Staphylococcus aureus. |
リンク | Structure / PubMed:20947019 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 7.9 - 13.4 Å |
構造データ | EMDB-1736: EMDB-1737: EMDB-1738: EMDB-1741: EMDB-1742: EMDB-1743: EMDB-1744: |
由来 |
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