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TitleCrystal Structure of Hexokinase KlHxk1 of Kluyveromyces lactis: A MOLECULAR BASIS FOR UNDERSTANDING THE CONTROL OF YEAST HEXOKINASE FUNCTIONS VIA COVALENT MODIFICATION AND OLIGOMERIZATION.
Journal, issue, pagesJ. Biol. Chem., Vol. 285, Page 41019-41033, Year 2010
Publish dateJul 19, 2010 (structure data deposition date)
AuthorsKuettner, E.B. / Kettner, K. / Keim, A. / Svergun, D.I. / Volke, D. / Singer, D. / Hoffmann, R. / Muller, E.C. / Otto, A. / Kriegel, T.M. / Strater, N.
External linksJ. Biol. Chem. / PubMed:20943665
MethodsX-ray diffraction
Resolution1.42 - 2.8 Å
Structure data

PDB-3o08:
Crystal structure of dimeric KlHxk1 in crystal form I
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

PDB-3o1b:
CRYSTAL STRUCTURE OF DIMERIC KLHXK1 IN CRYSTAL FORM II
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-3o1w:
Crystal structure of dimeric KlHxk1 in crystal form III
Method: X-RAY DIFFRACTION / Resolution: 1.66 Å

PDB-3o4w:
Crystal structure of dimeric KlHxk1 in crystal form IV
Method: X-RAY DIFFRACTION / Resolution: 1.61 Å

PDB-3o5b:
Crystal structure of dimeric KlHxk1 in crystal form VII with glucose bound (open state)
Method: X-RAY DIFFRACTION / Resolution: 1.97 Å

PDB-3o6w:
Crystal structure of monomeric KlHxk1 in crystal form VIII (open state)
Method: X-RAY DIFFRACTION / Resolution: 1.48 Å

PDB-3o80:
Crystal structure of monomeric KlHxk1 in crystal form IX (open state)
Method: X-RAY DIFFRACTION / Resolution: 2.18 Å

PDB-3o8m:
Crystal structure of monomeric KlHxk1 in crystal form XI with glucose bound (closed state)
Method: X-RAY DIFFRACTION / Resolution: 1.42 Å

Chemicals

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-NHE:
2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID / pH buffer*YM / CHES (buffer)

ChemComp-HOH:
WATER / Water

ChemComp-GOL:
GLYCEROL / Glycerol

ChemComp-PO4:
PHOSPHATE ION / Phosphate

ChemComp-BGC:
beta-D-glucopyranose / Glucose

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-GLC:
alpha-D-glucopyranose / Glucose

ChemComp-CL:
Unknown entry / Chloride

Source
  • kluyveromyces lactis (yeast)
KeywordsTRANSFERASE / RNaseH-like fold / Hexokinase / Glycolysis / Glucose repression / ATP binding / Mig1 binding

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