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Structure paper

TitleStructure and Function of Sphingosine-1-Phosphate Lyase, a Key Enzyme of Sphingolipid Metabolism.
Journal, issue, pagesStructure, Vol. 18, Page 1054-1065, Year 2010
Publish dateMar 23, 2010 (structure data deposition date)
AuthorsBourquin, F. / Riezman, H. / Capitani, G. / Grutter, M.G.
External linksStructure / PubMed:20696404
MethodsX-ray diffraction
Resolution2 - 3.15 Å
Structure data

PDB-3mad:
Crystal structure of StSPL (symmetric form)
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-3maf:
Crystal structure of StSPL (asymmetric form)
Method: X-RAY DIFFRACTION / Resolution: 2.971 Å

PDB-3mau:
Crystal structure of StSPL in complex with phosphoethanolamine
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-3mbb:
Crystal structure of StSPL - apo form, after treatment with semicarbazide
Method: X-RAY DIFFRACTION / Resolution: 2.051 Å

PDB-3mc6:
Crystal structure of ScDPL1
Method: X-RAY DIFFRACTION / Resolution: 3.15 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION

ChemComp-HOH:
WATER

ChemComp-CL:
Unknown entry

ChemComp-PLR:
(5-HYDROXY-4,6-DIMETHYLPYRIDIN-3-YL)METHYL DIHYDROGEN PHOSPHATE

ChemComp-EXT:
{5-hydroxy-6-methyl-4-[(E)-{[2-(phosphonooxy)ethyl]imino}methyl]pyridin-3-yl}methyl dihydrogen phosphate

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-NA:
Unknown entry

Source
  • symbiobacterium thermophilum (bacteria)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsLYASE / carboxy-lyase activity / Pyridoxal phosphate / pyridoxyl phosphate

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