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Title | Anthrax toxin-neutralizing antibody reconfigures the protective antigen heptamer into a supercomplex. |
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Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 107, Issue 32, Page 14070-14074, Year 2010 |
Publish date | Aug 10, 2010 |
![]() | Mazdak Radjainia / Jae-Kyung Hyun / Clinton E Leysath / Stephen H Leppla / Alok K Mitra / ![]() |
PubMed Abstract | The tripartite protein exotoxin secreted by Bacillus anthracis, a major contributor to its virulence and anthrax pathogenesis, consists of binary complexes of the protective antigen (PA) heptamer ...The tripartite protein exotoxin secreted by Bacillus anthracis, a major contributor to its virulence and anthrax pathogenesis, consists of binary complexes of the protective antigen (PA) heptamer (PA63h), produced by proteolytic cleavage of PA, together with either lethal factor or edema factor. The mouse monoclonal anti-PA antibody 1G3 was previously shown to be a potent antidote that shares F(C) domain dependency with the human monoclonal antibody MDX-1303 currently under clinical development. Here we demonstrate that 1G3 instigates severe perturbation of the PA63h structure and creates a PA supercomplex as visualized by electron microscopy. This phenotype, produced by the unconventional mode of antibody action, highlights the feasibility for optimization of vaccines based on analogous structural modification of PA63h as an additional strategy for future remedies against anthrax. |
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Methods | EM (single particle) |
Resolution | 33.0 Å |
Structure data | ![]() EMDB-5215: |
Source |
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