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TitleBiological and Structural Characterization of the Mycobacterium Smegmatis Nitroreductase Nfnb, and its Role in Benzothiazinone Resistance
Journal, issue, pagesMol. Microbiol., Vol. 77, Page 1172-, Year 2010
Publish dateDec 3, 2009 (structure data deposition date)
AuthorsManina, G. / Bellinzoni, M. / Pasca, M.R. / Neres, J. / Milano, A. / Ribeiro, A.L. / Buroni, S. / Skovierova, H. / Dianiskova, P. / Mikusova, K. ...Manina, G. / Bellinzoni, M. / Pasca, M.R. / Neres, J. / Milano, A. / Ribeiro, A.L. / Buroni, S. / Skovierova, H. / Dianiskova, P. / Mikusova, K. / Marak, J. / Makarov, V. / Giganti, D. / Haouz, A. / Lucarelli, A.P. / Degiacomi, G. / Piazza, A. / Chiarelli, L.R. / De Rossi, E. / Salina, E. / Cole, S.T. / Alzari, P.M. / Riccardi, G.
External linksMol. Microbiol. / PubMed:20624223
MethodsX-ray diffraction
Resolution1.75 - 1.8 Å
Structure data

PDB-2wzv:
Crystal structure of the FMN-dependent nitroreductase NfnB from Mycobacterium smegmatis
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-2wzw:
Crystal structure of the FMN-dependent nitroreductase NfnB from Mycobacterium smegmatis in complex with NADPH
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-PO4:
PHOSPHATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Source
  • mycobacterium smegmatis (bacteria)
KeywordsOXIDOREDUCTASE / NITROREDUCTASE

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