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TitleAb initio high-resolution single-particle 3D reconstructions: the symmetry adapted functions way.
Journal, issue, pagesJ Struct Biol, Vol. 172, Issue 3, Page 253-260, Year 2010
Publish dateJul 3, 2010
AuthorsLeandro F Estrozi / Jorge Navaza /
PubMed AbstractA protocol to attain high-resolution single-particle reconstructions is presented. The protocol is the concatenation of two procedures: one to obtain an ab initio low-resolution reconstruction, the ...A protocol to attain high-resolution single-particle reconstructions is presented. The protocol is the concatenation of two procedures: one to obtain an ab initio low-resolution reconstruction, the other to determine a fixed point of the consecutive applications of fast projection matching and 3D reconstruction. It is a reciprocal space formulation where the Fourier coefficients of the 3D scattering density are expressed in terms of symmetry adapted functions and the 2D particle images are represented by their Fourier-Bessel transforms. The new protocol shows advantages in terms of speed and accuracy when compared to other methods currently in use. We illustrate its performance as applied to high-resolution cryo-electron micrographs of rotavirus.
External linksJ Struct Biol / PubMed:20599509
MethodsEM (single particle)
Resolution3.5 Å
Structure data

EMDB-1752:
3D reconstruction of the rotavirus VP6 trimer using the Fast Projection Matching (FPM) algorithm.
Method: EM (single particle) / Resolution: 3.5 Å

Source
  • Bovine rotavirus

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