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Title | Ab initio high-resolution single-particle 3D reconstructions: the symmetry adapted functions way. |
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Journal, issue, pages | J Struct Biol, Vol. 172, Issue 3, Page 253-260, Year 2010 |
Publish date | Jul 3, 2010 |
Authors | Leandro F Estrozi / Jorge Navaza / |
PubMed Abstract | A protocol to attain high-resolution single-particle reconstructions is presented. The protocol is the concatenation of two procedures: one to obtain an ab initio low-resolution reconstruction, the ...A protocol to attain high-resolution single-particle reconstructions is presented. The protocol is the concatenation of two procedures: one to obtain an ab initio low-resolution reconstruction, the other to determine a fixed point of the consecutive applications of fast projection matching and 3D reconstruction. It is a reciprocal space formulation where the Fourier coefficients of the 3D scattering density are expressed in terms of symmetry adapted functions and the 2D particle images are represented by their Fourier-Bessel transforms. The new protocol shows advantages in terms of speed and accuracy when compared to other methods currently in use. We illustrate its performance as applied to high-resolution cryo-electron micrographs of rotavirus. |
External links | J Struct Biol / PubMed:20599509 |
Methods | EM (single particle) |
Resolution | 3.5 Å |
Structure data | EMDB-1752: |
Source |
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