+Search query
-Structure paper
Title | Macromolecular crystal data phased by negative-stained electron-microscopy reconstructions. |
---|---|
Journal, issue, pages | Acta Crystallogr D Biol Crystallogr, Vol. 66, Issue Pt 5, Page 514-521, Year 2010 |
Publish date | Apr 21, 2010 |
Authors | Stefano Trapani / Guy Schoehn / Jorge Navaza / Chantal Abergel / |
PubMed Abstract | The combination of transmission electron microscopy with X-ray diffraction data is usually limited to relatively large particles. Here, the approach is continued one step further by utilizing ...The combination of transmission electron microscopy with X-ray diffraction data is usually limited to relatively large particles. Here, the approach is continued one step further by utilizing negative staining, a technique that is of wider applicability than cryo-electron microscopy, to produce models of medium-size proteins suitable for molecular replacement. The technique was used to solve the crystal structure of the dodecameric type II dehydroquinase enzyme from Candida albicans (approximately 190 kDa) and that of the orthologous Streptomyces coelicolor protein. |
External links | Acta Crystallogr D Biol Crystallogr / PubMed:20445226 |
Methods | EM (single particle) / X-ray diffraction |
Resolution | 2.95 - 15.0 Å |
Structure data | EMDB-1680: PDB-3kip: |
Chemicals | ChemComp-SO4: ChemComp-TRS: ChemComp-HOH: |
Source |
|
Keywords | LYASE |