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-Structure paper
タイトル | Macromolecular crystal data phased by negative-stained electron-microscopy reconstructions. |
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ジャーナル・号・ページ | Acta Crystallogr D Biol Crystallogr, Vol. 66, Issue Pt 5, Page 514-521, Year 2010 |
掲載日 | 2010年4月21日 |
著者 | Stefano Trapani / Guy Schoehn / Jorge Navaza / Chantal Abergel / |
PubMed 要旨 | The combination of transmission electron microscopy with X-ray diffraction data is usually limited to relatively large particles. Here, the approach is continued one step further by utilizing ...The combination of transmission electron microscopy with X-ray diffraction data is usually limited to relatively large particles. Here, the approach is continued one step further by utilizing negative staining, a technique that is of wider applicability than cryo-electron microscopy, to produce models of medium-size proteins suitable for molecular replacement. The technique was used to solve the crystal structure of the dodecameric type II dehydroquinase enzyme from Candida albicans (approximately 190 kDa) and that of the orthologous Streptomyces coelicolor protein. |
リンク | Acta Crystallogr D Biol Crystallogr / PubMed:20445226 |
手法 | EM (単粒子) / X線回折 |
解像度 | 2.95 - 15.0 Å |
構造データ | EMDB-1680: PDB-3kip: |
化合物 | ChemComp-SO4: ChemComp-TRS: ChemComp-HOH: |
由来 |
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キーワード | LYASE (リアーゼ) |