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Structure paper

TitleStructures of an Isopenicillin N Converting Ntn-Hydrolase Reveal Different Catalytic Roles for the Active Site Residues of Precursor and Mature Enzyme.
Journal, issue, pagesStructure, Vol. 18, Page 301-, Year 2010
Publish dateDec 23, 2009 (structure data deposition date)
AuthorsBokhove, M. / Yoshida, H. / Hensgens, C.M.H. / Van Der Laan, J.M. / Sutherland, J.D. / Dijkstra, B.W.
External linksStructure / PubMed:20223213
MethodsX-ray diffraction
Resolution1.64 - 2 Å
Structure data

PDB-2x1c:
The crystal structure of precursor acyl coenzyme A:isopenicillin N acyltransferase from Penicillium chrysogenum
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-2x1d:
The crystal structure of mature acyl coenzyme A:isopenicillin N acyltransferase from Penicillium chrysogenum
Method: X-RAY DIFFRACTION / Resolution: 1.64 Å

PDB-2x1e:
The crystal structure of mature acyl coenzyme A:isopenicillin N acyltransferase from Penicillium chrysogenum in complex 6- aminopenicillanic acid
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-PO4:
PHOSPHATE ION

ChemComp-ACT:
ACETATE ION

ChemComp-D1D:
(4S,5S)-1,2-DITHIANE-4,5-DIOL

ChemComp-X1E:
(2S,5R,6R)-6-AMINO-3,3-DIMETHYL-7-OXO-4-THIA-1-AZABICYCLO[3.2.0]HEPTANE-2-CARBOXYLIC ACID / antibiotic*YM

Source
  • penicillium chrysogenum (fungus)
KeywordsTRANSFERASE / ZYMOGEN / NTN-HYDROLASE / PENICILLIN BIOSYNTHESIS / ACYLTRANSFERASE / ANTIBIOTIC BIOSYNTHESIS

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