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Structure paper

TitleMolecular basis for the high-affinity binding and stabilization of firefly luciferase by PTC124.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 107, Page 4878-4883, Year 2010
Publish dateJul 23, 2009 (structure data deposition date)
AuthorsAuld, D.S. / Lovell, S. / Thorne, N. / Lea, W.A. / Maloney, D.J. / Shen, M. / Rai, G. / Battaile, K.P. / Thomas, C.J. / Simeonov, A. ...Auld, D.S. / Lovell, S. / Thorne, N. / Lea, W.A. / Maloney, D.J. / Shen, M. / Rai, G. / Battaile, K.P. / Thomas, C.J. / Simeonov, A. / Hanzlik, R.P. / Inglese, J.
External linksProc. Natl. Acad. Sci. USA / PubMed:20194791
MethodsX-ray diffraction
Resolution2 - 2.1 Å
Structure data

PDB-3iep:
Firefly luciferase apo structure (P41 form)
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-3ier:
Firefly luciferase apo structure (P41 form) with PEG 400 bound
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

PDB-3ies:
Firefly luciferase inhibitor complex
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-M24:
5'-O-[(R)-[({3-[5-(2-fluorophenyl)-1,2,4-oxadiazol-3-yl]phenyl}carbonyl)oxy](hydroxy)phosphoryl]adenosine

Source
  • photinus pyralis (common eastern firefly)
KeywordsOXIDOREDUCTASE / MONOOXYGENASE / PHOTOPROTEIN / LUMINESCENCE / ATP-binding / Magnesium / Metal-binding / Nucleotide-binding / Peroxisome

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