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Structure paper

TitleUse of stabilizing mutations to engineer a charged group within a ligand-binding hydrophobic cavity in T4 lysozyme.
Journal, issue, pagesBiochemistry, Vol. 48, Page 8842-8851, Year 2009
Publish dateMar 30, 2009 (structure data deposition date)
AuthorsLiu, L. / Baase, W.A. / Michael, M.M. / Matthews, B.W.
External linksBiochemistry / PubMed:19663503
MethodsX-ray diffraction
Resolution1.45 - 2.24 Å
Structure data

PDB-3gui:
T4 lysozyme M102E/L99A mutant with buried charge in apolar cavity--Apo structure
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

PDB-3guj:
T4 lysozyme M102E/L99A mutant with buried charge in apolar cavity--Benzene binding
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-3guk:
T4 lysozyme M102E/L99A mutant with buried charge in apolar cavity--Toluene binding
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-3gul:
T4 lysozyme M102E/L99A mutant with buried charge in apolar cavity--ethylbenzene binding
Method: X-RAY DIFFRACTION / Resolution: 2.07 Å

PDB-3gum:
T4 lysozyme M102E/L99A mutant with buried charge in apolar cavity--p-xylene binding
Method: X-RAY DIFFRACTION / Resolution: 2.24 Å

PDB-3gun:
T4 lysozyme M102E/L99A mutant with buried charge in apolar cavity--aniline binding
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-3guo:
T4 lysozyme M102E/L99A mutant with buried charge in apolar cavity--phenol binding
Method: X-RAY DIFFRACTION / Resolution: 2.16 Å

PDB-3gup:
T4 lysozyme M102E/L99A mutant with buried charge in apolar cavity--pyridine binding
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

Chemicals

ChemComp-BME:
BETA-MERCAPTOETHANOL / 2-Mercaptoethanol

ChemComp-CO3:
CARBONATE ION / Carbonate

ChemComp-TAM:
TRIS(HYDROXYETHYL)AMINOMETHANE / pH buffer*YM / Tris

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-HOH:
WATER / Water

ChemComp-BNZ:
BENZENE / Benzene

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM / HEPES

ChemComp-MBN:
TOLUENE / Toluene

ChemComp-CA:
Unknown entry

ChemComp-CL:
Unknown entry / Chloride

ChemComp-ACT:
ACETATE ION / Acetate

ChemComp-PYJ:
PHENYLETHANE / Ethylbenzene

ChemComp-PXY:
PARA-XYLENE / P-Xylene

ChemComp-ANL:
ANILINE / Aniline

ChemComp-IPH:
PHENOL / Phenol

ChemComp-0PY:
pyridine / Pyridine

ChemComp-MG:
Unknown entry

Source
  • enterobacteria phage t4 (virus)
KeywordsHYDROLASE / T4 lysozyme / apolar cavity / buried charge / ligand binding / Antimicrobial / Bacteriolytic enzyme / Glycosidase

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