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TitleThe 3-dimensional structure of a hepatitis C virus p7 ion channel by electron microscopy.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 106, Issue 31, Page 12712-12716, Year 2009
Publish dateAug 4, 2009
AuthorsPhilipp Luik / Chee Chew / Jussi Aittoniemi / Jason Chang / Paul Wentworth / Raymond A Dwek / Philip C Biggin / Catherine Vénien-Bryan / Nicole Zitzmann /
PubMed AbstractInfection with the hepatitis C virus (HCV) has a huge impact on global health putting more than 170 million people at risk of developing severe liver disease. The HCV encoded p7 ion channel is ...Infection with the hepatitis C virus (HCV) has a huge impact on global health putting more than 170 million people at risk of developing severe liver disease. The HCV encoded p7 ion channel is essential for the production of infectious viruses. Despite a growing body of functional data, little is known about the 3-dimensional (3D) structure of the channel. Here, we present the 3D structure of a full-length viroporin, the detergent-solubilized hexameric 42 kDa form of the HCV p7 ion channel, as determined by single-particle electron microscopy using the random conical tilting approach. The reconstruction of such a small protein complex was made possible by a combination of high-contrast staining, the symmetry, and the distinct structural features of the channel. The orientation of the p7 monomers within the density was established using immunolabeling with N and C termini specific F(ab) fragments. The density map at a resolution of approximately 16 A reveals a flower-shaped protein architecture with protruding petals oriented toward the ER lumen. This broadest part of the channel presents a comparatively large surface area providing potential interaction sites for cellular and virally encoded ER resident proteins.
External linksProc Natl Acad Sci U S A / PubMed:19590017 / PubMed Central
MethodsEM (single particle)
Resolution16.0 Å
Structure data

EMDB-1661:
The three-dimensional structure of a hepatitis C virus p7 ion channel by electron microscopy
Method: EM (single particle) / Resolution: 16.0 Å

Source
  • Hepatitis C virus

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