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-Structure paper
| タイトル | Single-particle cryo-electron microscopy of Rift Valley fever virus. |
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| ジャーナル・号・ページ | Virology, Vol. 387, Issue 1, Page 11-15, Year 2009 |
| 掲載日 | 2009年4月25日 |
著者 | Michael B Sherman / Alexander N Freiberg / Michael R Holbrook / Stanley J Watowich / ![]() |
| PubMed 要旨 | Rift Valley fever virus (RVFV; Bunyaviridae; Phlebovirus) is an emerging human and veterinary pathogen causing acute hepatitis in ruminants and has the potential to cause hemorrhagic fever in humans. ...Rift Valley fever virus (RVFV; Bunyaviridae; Phlebovirus) is an emerging human and veterinary pathogen causing acute hepatitis in ruminants and has the potential to cause hemorrhagic fever in humans. We report a three-dimensional reconstruction of RVFV vaccine strain MP-12 (RVFV MP-12) by cryo-electron microcopy using icosahedral symmetry of individual virions. Although the genomic core of RVFV MP-12 is apparently poorly ordered, the glycoproteins on the virus surface are highly symmetric and arranged on a T=12 icosahedral lattice. Our RVFV MP-12 structure allowed clear identification of inter-capsomer contacts and definition of possible glycoprotein arrangements within capsomers. This structure provides a detailed model for phleboviruses, opens new avenues for high-resolution structural studies of the bunyavirus family, and aids the design of antiviral diagnostics and effective subunit vaccines. |
リンク | Virology / PubMed:19304307 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 27.0 Å |
| 構造データ | ![]() EMDB-5124: |
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