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-Structure paper
Title | Single-particle cryo-electron microscopy of Rift Valley fever virus. |
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Journal, issue, pages | Virology, Vol. 387, Issue 1, Page 11-15, Year 2009 |
Publish date | Apr 25, 2009 |
Authors | Michael B Sherman / Alexander N Freiberg / Michael R Holbrook / Stanley J Watowich / |
PubMed Abstract | Rift Valley fever virus (RVFV; Bunyaviridae; Phlebovirus) is an emerging human and veterinary pathogen causing acute hepatitis in ruminants and has the potential to cause hemorrhagic fever in humans. ...Rift Valley fever virus (RVFV; Bunyaviridae; Phlebovirus) is an emerging human and veterinary pathogen causing acute hepatitis in ruminants and has the potential to cause hemorrhagic fever in humans. We report a three-dimensional reconstruction of RVFV vaccine strain MP-12 (RVFV MP-12) by cryo-electron microcopy using icosahedral symmetry of individual virions. Although the genomic core of RVFV MP-12 is apparently poorly ordered, the glycoproteins on the virus surface are highly symmetric and arranged on a T=12 icosahedral lattice. Our RVFV MP-12 structure allowed clear identification of inter-capsomer contacts and definition of possible glycoprotein arrangements within capsomers. This structure provides a detailed model for phleboviruses, opens new avenues for high-resolution structural studies of the bunyavirus family, and aids the design of antiviral diagnostics and effective subunit vaccines. |
External links | Virology / PubMed:19304307 / PubMed Central |
Methods | EM (single particle) |
Resolution | 27.0 Å |
Structure data | EMDB-5124: |