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Title | The 3D structure of villin as an unusual F-Actin crosslinker. |
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Journal, issue, pages | Structure, Vol. 16, Issue 12, Page 1882-1891, Year 2008 |
Publish date | Dec 10, 2008 |
Authors | Cheri M Hampton / Jun Liu / Dianne W Taylor / David J DeRosier / Kenneth A Taylor / |
PubMed Abstract | Villin is an F-actin nucleating, crosslinking, severing, and capping protein within the gelsolin superfamily. We have used electron tomography of 2D arrays of villin-crosslinked F-actin to generate ...Villin is an F-actin nucleating, crosslinking, severing, and capping protein within the gelsolin superfamily. We have used electron tomography of 2D arrays of villin-crosslinked F-actin to generate 3D images revealing villin's crosslinking structure. In these polar arrays, neighboring filaments are spaced 125.9 +/- 7.1 A apart, offset axially by 17 A, with one villin crosslink per actin crossover. More than 6500 subvolumes containing a single villin crosslink and the neighboring actin filaments were aligned and classified to produce 3D subvolume averages. Placement of a complete villin homology model into the average density reveals that full-length villin binds to different sites on F-actin from those used by other actin-binding proteins and villin's close homolog gelsolin. |
External links | Structure / PubMed:19081064 / PubMed Central |
Methods | EM (tomography) |
Resolution | 30.0 Å |
Structure data | EMDB-1491: EMDB-1496: EMDB-1497: EMDB-1537: EMDB-1538: EMDB-1539: |
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