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TitleStructure of ratcheted ribosomes with tRNAs in hybrid states.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 105, Issue 44, Page 16924-16927, Year 2008
Publish dateNov 4, 2008
AuthorsPatricia Julián / Andrey L Konevega / Sjors H W Scheres / Melisa Lázaro / David Gil / Wolfgang Wintermeyer / Marina V Rodnina / Mikel Valle /
PubMed AbstractDuring protein synthesis, tRNAs and mRNA move through the ribosome between aminoacyl (A), peptidyl (P), and exit (E) sites of the ribosome in a process called translocation. Translocation is ...During protein synthesis, tRNAs and mRNA move through the ribosome between aminoacyl (A), peptidyl (P), and exit (E) sites of the ribosome in a process called translocation. Translocation is accompanied by the displacement of the tRNAs on the large ribosomal subunit toward the hybrid A/P and P/E states and by a rotational movement (ratchet) of the ribosomal subunits relative to one another. So far, the structure of the ratcheted state has been observed only when translation factors were bound to the ribosome. Using cryo-electron microscopy and classification, we show here that ribosomes can spontaneously adopt a ratcheted conformation with tRNAs in their hybrid states. The peptidyl-tRNA molecule in the A/P state, which is visualized here, is not distorted compared with the A/A state except for slight adjustments of its acceptor end, suggesting that the displacement of the A-site tRNA on the 50S subunit is passive and is induced by the 30S subunit rotation. Simultaneous subunit ratchet and formation of the tRNA hybrid states precede and may promote the subsequent rapid and coordinated tRNA translocation on the 30S subunit catalyzed by elongation factor G.
External linksProc Natl Acad Sci U S A / PubMed:18971332 / PubMed Central
MethodsEM (single particle)
Resolution13.0 - 16.0 Å
Structure data

EMDB-1551:
Pretranslocation ribosome. Total population.
Method: EM (single particle) / Resolution: 13.0 Å

EMDB-1553:
Pretranslocation ribosome. Group after ML3D classification. Racheted ribosome with tRNAs in hybrid positions.
Method: EM (single particle) / Resolution: 16.0 Å

EMDB-1554:
Pretranslocation ribosome. Group after ML3D classification. Non-racheted and classical tRNA positions.
Method: EM (single particle) / Resolution: 13.0 Å

Source
  • Escherichia coli (E. coli)

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