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TitleFiber formation across the bacterial outer membrane by the chaperone/usher pathway.
Journal, issue, pagesCell, Vol. 133, Issue 4, Page 640-652, Year 2008
Publish dateMay 16, 2008
AuthorsHan Remaut / Chunyan Tang / Nadine S Henderson / Jerome S Pinkner / Tao Wang / Scott J Hultgren / David G Thanassi / Gabriel Waksman / Huilin Li /
PubMed AbstractGram-negative pathogens commonly exhibit adhesive pili on their surfaces that mediate specific attachment to the host. A major class of pili is assembled via the chaperone/usher pathway. Here, the ...Gram-negative pathogens commonly exhibit adhesive pili on their surfaces that mediate specific attachment to the host. A major class of pili is assembled via the chaperone/usher pathway. Here, the structural basis for pilus fiber assembly and secretion performed by the outer membrane assembly platform--the usher--is revealed by the crystal structure of the translocation domain of the P pilus usher PapC and single particle cryo-electron microscopy imaging of the FimD usher bound to a translocating type 1 pilus assembly intermediate. These structures provide molecular snapshots of a twinned-pore translocation machinery in action. Unexpectedly, only one pore is used for secretion, while both usher protomers are used for chaperone-subunit complex recruitment. The translocating pore itself comprises 24 beta strands and is occluded by a folded plug domain, likely gated by a conformationally constrained beta-hairpin. These structures capture the secretion of a virulence factor across the outer membrane of gram-negative bacteria.
External linksCell / PubMed:18485872 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution3.2 - 23.0 Å
Structure data

EMDB-5009:
A cryo-EM map of the FimD-tip complex, a bacterial surface pilus assembly intermediate in complex with the outer membrane secretion channel.
Method: EM (single particle) / Resolution: 23.0 Å

PDB-2vqi:
Structure of the P pilus usher (PapC) translocation pore
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

Chemicals

ChemComp-LDA:
LAURYL DIMETHYLAMINE-N-OXIDE / LDAO, detergent*YM / Lauryldimethylamine oxide

ChemComp-C8E:
(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE / C8E, detergent*YM

ChemComp-HOH:
WATER / Water

Source
  • escherichia coli (E. coli)
KeywordsTRANSPORT / TRANSMEMBRANE / OUTER MEMBRANE / USHER / P PILUS / MEMBRANE / FIMBRIUM / OM TRANSLOCATION PORE / PILUS ASSEMBLY PLATFORM

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