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TitleA Ternary Complex of Hydroxycinnamoyl-Coa Hydratase-Lyase (Hchl) with Acetyl-Coa and Vanillin Gives Insights Into Substrate Specificity and Mechanism.
Journal, issue, pagesBiochem. J., Vol. 414, Page 281-, Year 2008
Publish dateApr 29, 2008 (structure data deposition date)
AuthorsBennett, J.P. / Bertin, L.M. / Moulton, B. / Fairlamb, I.J.S. / Brzozowski, A.M. / Walton, N.J. / Grogan, G.
External linksBiochem. J. / PubMed:18479250
MethodsX-ray diffraction
Resolution1.9 - 2.22 Å
Structure data

PDB-2vss:
Wild-type Hydroxycinnamoyl-CoA hydratase lyase in complex with acetyl- CoA and vanillin
Method: X-RAY DIFFRACTION / Resolution: 2.22 Å

PDB-2vsu:
A ternary complex of Hydroxycinnamoyl-CoA Hydratase-Lyase (HCHL) with acetyl-Coenzyme A and vanillin gives insights into substrate specificity and mechanism.
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-ACO:
ACETYL COENZYME *A

ChemComp-V55:
4-hydroxy-3-methoxybenzaldehyde

ChemComp-HOH:
WATER

Source
  • pseudomonas fluorescens (bacteria)
KeywordsLYASE / ALDOLASE / CROTONASE / HYDRATASE

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