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TitleThe flavivirus precursor membrane-envelope protein complex: structure and maturation.
Journal, issue, pagesScience, Vol. 319, Issue 5871, Page 1830-1834, Year 2008
Publish dateMar 28, 2008
AuthorsLong Li / Shee-Mei Lok / I-Mei Yu / Ying Zhang / Richard J Kuhn / Jue Chen / Michael G Rossmann /
PubMed AbstractMany viruses go through a maturation step in the final stages of assembly before being transmitted to another host. The maturation process of flaviviruses is directed by the proteolytic cleavage of ...Many viruses go through a maturation step in the final stages of assembly before being transmitted to another host. The maturation process of flaviviruses is directed by the proteolytic cleavage of the precursor membrane protein (prM), turning inert virus into infectious particles. We have determined the 2.2 angstrom resolution crystal structure of a recombinant protein in which the dengue virus prM is linked to the envelope glycoprotein E. The structure represents the prM-E heterodimer and fits well into the cryo-electron microscopy density of immature virus at neutral pH. The pr peptide beta-barrel structure covers the fusion loop in E, preventing fusion with host cell membranes. The structure provides a basis for identifying the stages of its pH-directed conformational metamorphosis during maturation, ending with release of pr when budding from the host.
External linksScience / PubMed:18369147
MethodsX-ray diffraction / EM (single particle)
Resolution2.2 - 12.5 Å
Structure data

PDB-3c5x:
Crystal structure of the precursor membrane protein- envelope protein heterodimer from the dengue 2 virus at low pH
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-3c6d:
The pseudo-atomic structure of dengue immature virus
Method: ELECTRON MICROSCOPY / Resolution: 12.5 Å

PDB-3c6e:
Crystal structure of the precursor membrane protein- envelope protein heterodimer from the dengue 2 virus at neutral pH
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-HOH:
WATER / Water

ChemComp-NDG:
2-acetamido-2-deoxy-alpha-D-glucopyranose / N-Acetylglucosamine

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

Source
  • dengue virus 2 thailand/16681/84
  • dengue virus 2
  • dengue virus
KeywordsVIRAL PROTEIN / BETA BARREL / prM-E protein complex / Helicase / Hydrolase / Nucleotide-binding / RNA replication / Transmembrane / VIRUS / icosahedral virion / ATP-binding / Capsid protein / Cleavage on pair of basic residues / Endoplasmic reticulum / Envelope protein / Glycoprotein / Metal-binding / Multifunctional enzyme / Nucleotidyltransferase / Nucleus / Phosphoprotein / Protease / Ribonucleoprotein / RNA-binding / RNA-directed RNA polymerase / Secreted / Serine protease / Transcription / Transcription regulation / Transferase / Viral nucleoprotein / icosahedral virus / prM-E protein complex structure

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