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Structure paper

TitleCrystal Structure of a Type II Dihydrofolate Reductase Catalytic Ternary Complex
Journal, issue, pagesBiochemistry, Vol. 46, Page 14878-14888, Year 2007
Publish dateOct 5, 2007 (structure data deposition date)
AuthorsKrahn, J.M. / Jackson, M.R. / DeRose, E.F. / Howell, E.E. / London, R.E.
External linksBiochemistry / PubMed:18052202
MethodsX-ray diffraction
Resolution0.96 - 1.9 Å
Structure data

PDB-2rh2:
High Resolution DHFR R-67
Method: X-RAY DIFFRACTION / Resolution: 0.96 Å

PDB-2rk1:
DHFR R67 Complexed with NADP and dihydrofolate
Method: X-RAY DIFFRACTION / Resolution: 1.26 Å

PDB-2rk2:
DHFR R-67 complexed with NADP
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-MRD:
(4R)-2-METHYLPENTANE-2,4-DIOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-DHF:
DIHYDROFOLIC ACID

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Source
  • escherichia coli (E. coli)
KeywordsOXIDOREDUCTASE / FOLATE METABOLISM / PLASMID-ENCODED R67 DHFR / TMP-RESISTANT DHFR / Antibiotic resistance / Methotrexate resistance / NADP / One-carbon metabolism / Trimethoprim resistance / dihydrofolate / asymmetric ligand binding / Plasmid

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