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TitleCrystal structure, stability and in vitro RNAi activity of oligoribonucleotides containing the ribo-difluorotoluyl nucleotide: insights into substrate requirements by the human RISC Ago2 enzyme.
Journal, issue, pagesNucleic Acids Res., Vol. 35, Page 6424-6438, Year 2007
Publish dateMay 25, 2007 (structure data deposition date)
AuthorsLi, F. / Pallan, P.S. / Maier, M.A. / Rajeev, K.G. / Mathieu, S.L. / Kreutz, C. / Fan, Y. / Sanghvi, J. / Micura, R. / Rozners, E. ...Li, F. / Pallan, P.S. / Maier, M.A. / Rajeev, K.G. / Mathieu, S.L. / Kreutz, C. / Fan, Y. / Sanghvi, J. / Micura, R. / Rozners, E. / Manoharan, M. / Egli, M.
External linksNucleic Acids Res. / PubMed:17881374
MethodsX-ray diffraction
Resolution1.1 - 1.12 Å
Structure data

PDB-2q1o:
Crystal Structure Analysis of the RNA Dodecamer CGC-NF2-AAUUGGCG, with an Incorporated 2,4-Difluorotoluyl Residue (NF2)
Method: X-RAY DIFFRACTION / Resolution: 1.1 Å

PDB-2q1r:
Crystal Structure Analysis of the RNA Dodecamer CGCGAAUUAGCG, with a G-A mismatch.
Method: X-RAY DIFFRACTION / Resolution: 1.12 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

KeywordsRNA / 2 / 4-Difluorotoluyl Nucleoside / Chemical Modification / RNAi / Hydrogen Bond / G-A mismatch

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