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Structure paper

TitleCrystal structure of lactaldehyde dehydrogenase from Escherichia coli and inferences regarding substrate and cofactor specificity
Journal, issue, pagesJ. Mol. Biol., Vol. 366, Page 481-493, Year 2007
Publish dateJun 26, 2006 (structure data deposition date)
AuthorsDi Costanzo, L. / Gomez, G.A. / Christianson, D.W.
External linksJ. Mol. Biol. / PubMed:17173928
MethodsX-ray diffraction
Resolution2.1 - 2.7 Å
Structure data

PDB-2hg2:
Structure of Lactaldehyde Dehydrogenase
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-2ilu:
Crystal structure of lactaldehyde dehydrogenase from E. coli: the binary complex with NADPH
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-2imp:
Crystal structure of lactaldehyde dehydrogenase from E. coli: the ternary complex with lactate (occupancy 0.5) and NADH. Crystals soaked with (L)-Lactate.
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-NAI:
1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE

ChemComp-LAC:
LACTIC ACID

Source
  • escherichia coli (E. coli)
KeywordsOXIDOREDUCTASE / dehydrogenase / NAD dependent / NADPH-Lactaldehyde dehydrogenase complex / protein-lactate-NADH ternary complex

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