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-Structure paper
タイトル | Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM. |
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ジャーナル・号・ページ | Structure, Vol. 14, Issue 11, Page 1655-1665, Year 2006 |
掲載日 | 2007年1月16日 |
![]() | Lu Gan / Jeffrey A Speir / James F Conway / Gabriel Lander / Naiqian Cheng / Brian A Firek / Roger W Hendrix / Robert L Duda / Lars Liljas / John E Johnson / ![]() |
PubMed 要旨 | Maturation of the bacteriophage HK97 capsid from a precursor (Prohead II) to the mature state (Head II) involves a 60 A radial expansion. The mature particle is formed by 420 copies of the major ...Maturation of the bacteriophage HK97 capsid from a precursor (Prohead II) to the mature state (Head II) involves a 60 A radial expansion. The mature particle is formed by 420 copies of the major capsid protein organized on a T = 7 laevo lattice with each subunit covalently crosslinked to two neighbors. Well-characterized pH 4 expansion intermediates make HK97 valuable for investigating quaternary structural dynamics. Here, we use X-ray crystallography and cryo-EM to demonstrate that in the final transition in maturation (requiring neutral pH), pentons in Expansion Intermediate IV (EI-IV) reversibly sample 14 A translations and 6 degrees rotations relative to a fixed hexon lattice. The limit of this trajectory corresponds to the Head II conformation that is secured at this extent only by the formation of the final class of covalent crosslinks. Mutants that cannot crosslink or EI-IV particles that have been rendered incapable of forming the final crosslink remain in the EI-IV state. |
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手法 | X線回折 / EM (単粒子) |
解像度 | 3.8 - 7.5 Å |
構造データ | ![]() PDB-2frp: ![]() PDB-2fs3: ![]() PDB-2fsy: ![]() PDB-2ft1: ![]() PDB-2fte: |
由来 |
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![]() | VIRUS / Bacteriophage / HK97 / Capsid Protein / Expansion Intermediate / icosahedral VIRUS / capsid / mature capsid / VIRUS/VIRAL PROTEIN / VIRUS-VIRAL PROTEIN COMPLEX |