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-Structure paper
| タイトル | Organization of core spliceosomal components U5 snRNA loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as revealed by electron cryomicroscopy. |
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| ジャーナル・号・ページ | Mol Cell, Vol. 24, Issue 2, Page 267-278, Year 2006 |
| 掲載日 | 2006年10月20日 |
著者 | Bjoern Sander / Monika M Golas / Evgeny M Makarov / Hero Brahms / Berthold Kastner / Reinhard Lührmann / Holger Stark / ![]() |
| PubMed 要旨 | In eukaryotes, pre-mRNA exons are interrupted by large noncoding introns. Alternative selection of exons and nucleotide-exact removal of introns are performed by the spliceosome, a highly dynamic ...In eukaryotes, pre-mRNA exons are interrupted by large noncoding introns. Alternative selection of exons and nucleotide-exact removal of introns are performed by the spliceosome, a highly dynamic macromolecular machine. U4/U6.U5 tri-snRNP is the largest and most conserved building block of the spliceosome. By 3D electron cryomicroscopy and labeling, the exon-aligning U5 snRNA loop I is localized at the center of the tetrahedrally shaped tri-snRNP reconstructed to approximately 2.1 nm resolution in vitrified ice. Independent 3D reconstructions of its subunits, U4/U6 and U5 snRNPs, show how U4/U6 and U5 combine to form tri-snRNP and, together with labeling experiments, indicate a close proximity of the spliceosomal core components U5 snRNA loop I and U4/U6 at the center of tri-snRNP. We suggest that this central tri-snRNP region may be the site to which the prespliceosomal U2 snRNA has to approach closely during formation of the catalytic core of the spliceosome. |
リンク | Mol Cell / PubMed:17052460 |
| 手法 | EM (単粒子) |
| 解像度 | 24.0 - 40.0 Å |
| 構造データ | ![]() EMDB-1257: ![]() EMDB-1258: ![]() EMDB-1259: |
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Homo sapiens (ヒト)