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Structure paper

TitleBasic requirements for a metal-binding site in a protein: The influence of loop shortening on the cupredoxin azurin.
Journal, issue, pagesProc. Natl. Acad. Sci. Usa, Vol. 103, Page 7258-7263, Year 2006
Publish dateJan 24, 2006 (structure data deposition date)
AuthorsLi, C. / Yanagisawa, S. / Martins, B.M. / Messerschmidt, A. / Banfield, M.J. / Dennison, C.
External linksProc. Natl. Acad. Sci. Usa / PubMed:16651527
MethodsX-ray diffraction
Resolution1.25 - 1.61 Å
Structure data

PDB-2ft6:
Structure of Cu(II)azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CTPHPM"
Method: X-RAY DIFFRACTION / Resolution: 1.25 Å

PDB-2ft7:
Structure of Cu(I)azurin at pH 6, with the metal-binding loop sequence "CTFPGHSALM" replaced with "CTPHPM"
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-2ft8:
Structure of Cu(I)azurin, pH8, with the metal-binding loop sequence "CTFPGHSALM" replaced with "CTPHPM"
Method: X-RAY DIFFRACTION / Resolution: 1.55 Å

PDB-2fta:
Structure of Cu(II)azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CTPHPFM"
Method: X-RAY DIFFRACTION / Resolution: 1.61 Å

Chemicals

ChemComp-CU:
COPPER (II) ION

ChemComp-HOH:
WATER

ChemComp-CU1:
COPPER (I) ION

ChemComp-2PE:
NONAETHYLENE GLYCOL / precipitant*YM

ChemComp-EOH:
ETHANOL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • pseudomonas aeruginosa (bacteria)
KeywordsELECTRON TRANSPORT / Blue copper-binding protein / greek-key beta-barrel / greek-key beta-barrel.

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