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TitleMolecular Recognition of the Substrate Diphosphate Group Governs Product Diversity in Trichodiene Synthase Mutants.
Journal, issue, pagesBiochemistry, Vol. 44, Page 6153-6163, Year 2005
Publish dateJan 13, 2005 (structure data deposition date)
AuthorsVedula, L.S. / Rynkiewicz, M.J. / Pyun, H.J. / Coates, R.M. / Cane, D.E. / Christianson, D.W.
External linksBiochemistry / PubMed:15835903
MethodsX-ray diffraction
Resolution2.1 - 2.95 Å
Structure data

PDB-1yj4:
Y305F Trichodiene Synthase
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-1yyq:
Y305F Trichodiene Synthase complexed with pyrophosphate
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-1yyr:
Y305F Trichodiene Synthase: Complex With Mg, Pyrophosphate, and (4R)-7-azabisabolene
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-1yys:
Y305F Trichodiene Synthase: Complex With Mg, Pyrophosphate, and (4S)-7-azabisabolene
Method: X-RAY DIFFRACTION / Resolution: 2.75 Å

PDB-1yyt:
D100E Trichodiene Synthase: Complex With Mg, Pyrophosphate, and (4R)-7-azabisabolene
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-1yyu:
D100E Trichodiene Synthase: Complex With Mg, Pyrophosphate, and (4S)-7-azabisabolene
Method: X-RAY DIFFRACTION / Resolution: 2.95 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-POP:
PYROPHOSPHATE 2-

ChemComp-SAZ:
(1S)-N,4-DIMETHYL-N-(4-METHYLPENT-3-ENYL)CYCLOHEX-3-ENAMINIUM

ChemComp-RAZ:
(1R)-N,4-DIMETHYL-N-(4-METHYLPENT-3-ENYL)CYCLOHEX-3-ENAMINIUM

Source
  • fusarium sporotrichioides (fungus)
KeywordsLYASE / terpenoid cyclase fold / site-directed mutant / Pyrophosphate / (4R)-7-azabisabolene / (4S)-7-azabisabolene

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