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Structure paper

TitleInteraction of phosphonate analogues of the tetrahedral reaction intermediate with 5-enolpyruvylshikimate-3-phosphate synthase in atomic detail.
Journal, issue, pagesBiochemistry, Vol. 44, Page 3241-3248, Year 2005
Publish dateAug 18, 2004 (structure data deposition date)
AuthorsPriestman, M.A. / Healy, M.L. / Becker, A. / Alberg, D.G. / Bartlett, P.A. / Lushington, G.H. / Schonbrunn, E.
External linksBiochemistry / PubMed:15736934
MethodsX-ray diffraction
Resolution1.5 - 1.9 Å
Structure data

PDB-1x8r:
EPSPS liganded with the (S)-phosphonate analog of the tetrahedral reaction intermediate
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-1x8t:
EPSPS liganded with the (R)-phosphonate analog of the tetrahedral reaction intermediate
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-SC1:
[3R-[3A,4A,5B(S*)]]-5-(1-CARBOXY-1-PHOSPHONOETHOXY)-4-HYDROXY-3-(PHOSPHONOOXY)-1-CYCLOHEXENE-1-CARBOXYLIC ACID

ChemComp-FMT:
FORMIC ACID

ChemComp-HOH:
WATER

ChemComp-RC1:
[3R-[3A,4A,5B(R*)]]-5-(1-CARBOXY-1-PHOSPHONOETHOXY)-4-HYDROXY-3-(PHOSPHONOOXY)-1-CYCLOHEXENE-1-CARBOXYLIC ACID

Source
  • escherichia coli (E. coli)
KeywordsTRANSFERASE / inside-out alpha-beta barrel

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