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TitleObserving translesion synthesis of an aromatic amine DNA adduct by a high-fidelity DNA polymerase
Journal, issue, pagesJ. Biol. Chem., Vol. 279, Page 50280-50285, Year 2004
Publish dateAug 11, 2004 (structure data deposition date)
AuthorsHsu, G.W. / Kiefer, J.R. / Burnouf, D. / Becherel, O.J. / Fuchs, R.P.P. / Beese, L.S.
External linksJ. Biol. Chem. / PubMed:15385534
MethodsX-ray diffraction
Resolution2 - 2.1 Å
Structure data

PDB-1ua0:
Aminofluorene DNA adduct at the pre-insertion site of a DNA polymerase
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-1ua1:
Structure of aminofluorene adduct paired opposite cytosine at the polymerase active site.
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-AF:
2-AMINOFLUORENE

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

Source
  • geobacillus stearothermophilus (bacteria)
KeywordsTRANSFERASE/DNA / DNA polymerase I; DNA replication; klenow fragment; protein-DNA complex; aminofluorene; aromatic amine; DNA lesion; translation replication / TRANSFERASE-DNA COMPLEX

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