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TitleCrystal Complex Structures Reveal How Substrate is Bound in the -4 to the +2 Binding Sites of Humicola Grisea Cel12A
Journal, issue, pagesJ. Mol. Biol., Vol. 342, Page 1505-, Year 2004
Publish dateDec 15, 2003 (structure data deposition date)
AuthorsSandgren, M. / Berglund, G.I. / Shaw, A. / Stahlberg, J. / Kenne, L. / Desmet, T. / Mitchinson, C.
External linksJ. Mol. Biol. / PubMed:15364577
MethodsX-ray diffraction
Resolution1.4 - 1.67 Å
Structure data

PDB-1uu4:
X-RAY CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF HUMICOLA GRISEA CEL12A IN COMPLEX WITH CELLOBIOSE
Method: X-RAY DIFFRACTION / Resolution: 1.49 Å

PDB-1uu5:
X-RAY CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF HUMICOLA GRISEA CEL12A SOAKED WITH CELLOTETRAOSE
Method: X-RAY DIFFRACTION / Resolution: 1.67 Å

PDB-1uu6:
X-RAY CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF HUMICOLA GRISEA CEL12A IN COMPLEX WITH A SOAKED CELLOPENTAOSE
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-1w2u:
X-RAY CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF HUMICOLA GRISEA CEL12A IN COMPLEX WITH A SOAKED THIO CELLOTETRAOSE
Method: X-RAY DIFFRACTION / Resolution: 1.52 Å

Chemicals

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-ACT:
ACETATE ION

ChemComp-SO4:
SULFATE ION

Source
  • humicola grisea (fungus)
KeywordsHYDROLASE / CELLULASE / CELLULOSE DEGRADATION / ENDOGLUCANASE / GLYCOSYL HYDROLASE / GH FAMILY 12 / HUMICOLA GRISEA CEL12A / LIGAND COMPLEX

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