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-Structure paper
タイトル | A 9 angstroms single particle reconstruction from CCD captured images on a 200 kV electron cryomicroscope. |
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ジャーナル・号・ページ | J Struct Biol, Vol. 147, Issue 2, Page 116-127, Year 2004 |
掲載日 | 2005年1月26日 |
![]() | Christopher R Booth / Wen Jiang / Matthew L Baker / Z Hong Zhou / Steven J Ludtke / Wah Chiu / ![]() |
PubMed 要旨 | Sub-nanometer resolution structure determination is becoming a common practice in electron cryomicroscopy of macromolecular assemblies. The data for these studies have until now been collected on ...Sub-nanometer resolution structure determination is becoming a common practice in electron cryomicroscopy of macromolecular assemblies. The data for these studies have until now been collected on photographic film. Using cytoplasmic polyhedrosis virus (CPV), a previously determined structure, as a test specimen, we show the feasibility of obtaining a 9 angstroms structure from images acquired from a 4 k x 4 k Gatan CCD on a 200 kV electron cryomicroscope. The match of the alpha-helices in the protein components of the CPV with the previous structure of the same virus validates the suitability of this type of camera as the recording media targeted for single particle reconstructions at sub-nanometer resolution. |
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手法 | EM (単粒子) |
解像度 | 9.0 Å |
構造データ | ![]() EMDB-1165: |