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TitleCrystal structure of Pseudomonas fluorescens mannitol 2-dehydrogenase binary and ternary complexes. Specificity and catalytic mechanism
Journal, issue, pagesJ. Biol. Chem., Vol. 277, Page 43433-43442, Year 2002
Publish dateApr 19, 2002 (structure data deposition date)
AuthorsKavanagh, K.L. / Klimacek, M. / Nidetzky, B. / Wilson, D.K.
External linksJ. Biol. Chem. / PubMed:12196534
MethodsX-ray diffraction
Resolution1.7 - 1.8 Å
Structure data

PDB-1lj8:
Crystal structure of mannitol dehydrogenase in complex with NAD
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-1m2w:
Pseudomonas fluorescens mannitol 2-dehydrogenase ternary complex with NAD and D-mannitol
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-HOH:
WATER

ChemComp-MTL:
D-MANNITOL / medication*YM

Source
  • pseudomonas fluorescens (bacteria)
KeywordsOXIDOREDUCTASE / NAD / long-chain dehydrogenase / Rossmann fold / di-nucleotide binding motif / polyol dehydrogenase / secondary alcohol dehydrogenase

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