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TitleEffects of site-directed mutagenesis on structure and function of recombinant rat liver S-adenosylhomocysteine hydrolase. Crystal structure of D244E mutant enzyme.
Journal, issue, pagesJ. Biol. Chem., Vol. 275, Page 32147-32156, Year 2000
Publish dateJun 22, 2000 (structure data deposition date)
AuthorsKomoto, J. / Huang, Y. / Gomi, T. / Ogawa, H. / Takata, Y. / Fujioka, M. / Takusagawa, F.
External linksJ. Biol. Chem. / PubMed:10913437
MethodsX-ray diffraction
Resolution2.8 Å
Structure data

PDB-1d4f:
CRYSTAL STRUCTURE OF RECOMBINANT RAT-LIVER D244E MUTANT S-ADENOSYLHOMOCYSTEINE HYDROLASE
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

Chemicals

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-ADN:
ADENOSINE

ChemComp-HOH:
WATER

Source
  • rattus norvegicus (Norway rat)
KeywordsHYDROLASE / S-adenosylhomocysteine hydrolase / AdoHcyase / AdoHcy / Mutagenesis / X-ray crystal structure / Enzyme structure

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