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Structure paper

タイトル	Structural basis of BAX pore formation.
ジャーナル・号・ページ	Science, Vol. 388, Issue 6754, Page eadv4314, Year 2025
掲載日	2025年6月26日
著者	Ying Zhang / Lu Tian / Gaoxingyu Huang / Xiaofei Ge / Fang Kong / Pengqi Wang / Yige Xu / Yigong Shi /
PubMed 要旨	During apoptosis, cytosolic BAX monomers are translocated to the mitochondria to permeabilize the outer membrane. Here, we identified a dimer of BAX dimers as the basic repeating unit of its various ...During apoptosis, cytosolic BAX monomers are translocated to the mitochondria to permeabilize the outer membrane. Here, we identified a dimer of BAX dimers as the basic repeating unit of its various oligomeric forms: arcs, lines, and rings. Cryo-electron microscopy structure of the BAX repeating unit at 3.2-angstrom resolution revealed the interactions within and between dimers. End-to-end stacking of the repeating units through the protruding α9 pairs yielded lines, arcs, polygons, and rings. We structurally characterized the tetragon, pentagon, hexagon, and heptagon, which comprise 16, 20, 24, and 28 BAX protomers, respectively. Missense mutations at the BAX inter-protomer interface damage pore formation and cripple its proapoptotic function. The assembly principle of the various BAX oligomers reported here provides the structural basis of membrane permeabilization by BAX.
リンク	Science / PubMed:40570108
手法	EM (単粒子)
解像度	3.19 - 18.1 Å
構造データ	60977 9ixu EMDB-60977, PDB-9ixu: Overall reconstruction of the Bax line 手法: EM (単粒子) / 解像度: 3.19 Å EMDB-61005: Tetragon ring reconstruction of the BAX ring 手法: EM (単粒子) / 解像度: 8.8 Å EMDB-61007: Pentagon reconstruction of the BAX ring 手法: EM (単粒子) / 解像度: 9.6 Å EMDB-61010: Hexagon reconstruction of the BAX ring 手法: EM (単粒子) / 解像度: 9.6 Å EMDB-61017: Heptagonal reconstruction of the BAX ring 手法: EM (単粒子) / 解像度: 18.1 Å EMDB-61020: tetragon vertex reconstruction of the BAX ring 手法: EM (単粒子) / 解像度: 5.12 Å EMDB-61021: Pentagon vertex reconstruction of the BAX ring 手法: EM (単粒子) / 解像度: 5.02 Å EMDB-61022: Hexagon vertex reconstruction of the BAX ring 手法: EM (単粒子) / 解像度: 5.27 Å EMDB-61023: Heptagon vertex reconstruction of the BAX ring 手法: EM (単粒子) / 解像度: 5.53 Å
由来	homo sapiens (ヒト)
キーワード	APOPTOSIS / Pore forming Bax proteins