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キーワード
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

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Structure paper

タイトル	Polymerization of ZBTB transcription factors regulates chromatin occupancy.
ジャーナル・号・ページ	Mol Cell, Vol. 84, Issue 13, Page 2511-22524.e8, Year 2024
掲載日	2024年7月11日
著者	Paul M C Park / Jiho Park / Jared Brown / Moritz Hunkeler / Shourya S Roy Burman / Katherine A Donovan / Hojong Yoon / Radosław P Nowak / Mikołaj Słabicki / Benjamin L Ebert / Eric S Fischer /
PubMed 要旨	BCL6, an oncogenic transcription factor (TF), forms polymers in the presence of a small-molecule molecular glue that stabilizes a complementary interface between homodimers of BCL6's broad-complex, ...BCL6, an oncogenic transcription factor (TF), forms polymers in the presence of a small-molecule molecular glue that stabilizes a complementary interface between homodimers of BCL6's broad-complex, tramtrack, and bric-à-brac (BTB) domain. The BTB domains of other proteins, including a large class of TFs, have similar architectures and symmetries, raising the possibility that additional BTB proteins self-assemble into higher-order structures. Here, we surveyed 189 human BTB proteins with a cellular fluorescent reporter assay and identified 18 ZBTB TFs that show evidence of polymerization. Through biochemical and cryoelectron microscopy (cryo-EM) studies, we demonstrate that these ZBTB TFs polymerize into filaments. We found that BTB-domain-mediated polymerization of ZBTB TFs enhances chromatin occupancy within regions containing homotypic clusters of TF binding sites, leading to repression of target genes. Our results reveal a role of higher-order structures in regulating ZBTB TFs and suggest an underappreciated role for TF polymerization in modulating gene expression.
リンク	Mol Cell / PubMed:38996460 / PubMed Central
手法	EM (単粒子) / EM (らせん対称)
解像度	3.7 - 8.1 Å
構造データ	44389 9b9r EMDB-44389, PDB-9b9r: Cryo-EM structure of the ZBTB5 BTB domain filament 手法: EM (単粒子) / 解像度: 3.7 Å 44391 9b9v EMDB-44391, PDB-9b9v: Cryo-EM structure of the ZBTB9 BTB domain filament 手法: EM (らせん対称) / 解像度: 8.1 Å
由来	homo sapiens (ヒト)
キーワード	TRANSCRIPTION / BTB domain / transcription factor / ZBTB protein