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| Title | A viral RNA hijacks host machinery using dynamic conformational changes of a tRNA-like structure. |
|---|---|
| Journal, issue, pages | Science, Vol. 374, Issue 6570, Page 955-960, Year 2021 |
| Publish date | Nov 19, 2021 |
 Authors | Steve L Bonilla / Madeline E Sherlock / Andrea MacFadden / Jeffrey S Kieft /  |
| PubMed Abstract | Viruses require multifunctional structured RNAs to hijack their host’s biochemistry, but their mechanisms can be obscured by the difficulty of solving conformationally dynamic RNA structures. Using ...Viruses require multifunctional structured RNAs to hijack their host’s biochemistry, but their mechanisms can be obscured by the difficulty of solving conformationally dynamic RNA structures. Using cryo–electron microscopy (cryo-EM), we visualized the structure of the mysterious viral transfer RNA (tRNA)–like structure (TLS) from the brome mosaic virus, which affects replication, translation, and genome encapsidation. Structures in isolation and those bound to tyrosyl-tRNA synthetase (TyrRS) show that this ~55-kilodalton purported tRNA mimic undergoes large conformational rearrangements to bind TyrRS in a form that differs substantially from that of tRNA. Our study reveals how viral RNAs can use a combination of static and dynamic RNA structures to bind host machinery through highly noncanonical interactions, and we highlight the utility of cryo-EM for visualizing small, conformationally dynamic structured RNAs. |
 External links | Science / PubMed:34793227 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 4.3 - 6.0 Å |
| Structure data | EMDB-24952, PDB-7sam: EMDB-25023, PDB-7sc6: EMDB-25041, PDB-7scq: |
| Source |
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 Keywords | RNA / Viral RNA / 3' UTR / RNA BINDING PROTEIN/RNA / tRNA-synthetase / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA complex / RNA-protein complex |
brome mosaic virus
phaseolus vulgaris (French bean)
