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Structure paper

TitleStructure, function and pharmacology of human itch GPCRs.
Journal, issue, pagesNature, Year 2021
Publish dateNov 17, 2021
AuthorsCan Cao / Hye Jin Kang / Isha Singh / He Chen / Chengwei Zhang / Wenlei Ye / Byron W Hayes / Jing Liu / Ryan H Gumpper / Brian J Bender / Samuel T Slocum / Brian E Krumm / Katherine Lansu / John D McCorvy / Wesley K Kroeze / Justin G English / Jeffrey F DiBerto / Reid H J Olsen / Xi-Ping Huang / Shicheng Zhang / Yongfeng Liu / Kuglae Kim / Joel Karpiak / Lily Y Jan / Soman N Abraham / Jian Jin / Brian K Shoichet / Jonathan F Fay / Bryan L Roth /
PubMed AbstractThe MRGPRX family of receptors (MRGPRX1-4) is a family of mas-related G-protein-coupled receptors that have evolved relatively recently. Of these, MRGPRX2 and MRGPRX4 are key physiological and ...The MRGPRX family of receptors (MRGPRX1-4) is a family of mas-related G-protein-coupled receptors that have evolved relatively recently. Of these, MRGPRX2 and MRGPRX4 are key physiological and pathological mediators of itch and related mast cell-mediated hypersensitivity reactions. MRGPRX2 couples to both G and G in mast cells. Here we describe agonist-stabilized structures of MRGPRX2 coupled to G and G in ternary complexes with the endogenous peptide cortistatin-14 and with a synthetic agonist probe, respectively, and the development of potent antagonist probes for MRGPRX2. We also describe a specific MRGPRX4 agonist and the structure of this agonist in a complex with MRGPRX4 and G. Together, these findings should accelerate the structure-guided discovery of therapeutic agents for pain, itch and mast cell-mediated hypersensitivity.
External linksNature / PubMed:34789874
MethodsEM (single particle)
Resolution2.45 - 2.9 Å
Structure data

EMDB-24896, PDB-7s8l:
CryoEM structure of Gq-coupled MRGPRX2 with peptide agonist Cortistatin-14
Method: EM (single particle) / Resolution: 2.45 Å

EMDB-24897, PDB-7s8m:
CryoEM structure of Gi-coupled MRGPRX2 with peptide agonist Cortistatin-14
Method: EM (single particle) / Resolution: 2.54 Å

EMDB-24898, PDB-7s8n:
CryoEM structure of Gq-coupled MRGPRX2 with small molecule agonist (R)-Zinc-3573
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-24899, PDB-7s8o:
CryoEM structure of Gi-coupled MRGPRX2 with small molecule agonist (R)-Zinc-3573
Method: EM (single particle) / Resolution: 2.58 Å

EMDB-24900, PDB-7s8p:
CryoEM structure of Gq-coupled MRGPRX4 with small molecule agonist MS47134
Method: EM (single particle) / Resolution: 2.6 Å

Chemicals

ChemComp-8IU:
(3R)-N,N-dimethyl-1-[(8S)-5-phenylpyrazolo[1,5-a]pyrimidin-7-yl]pyrrolidin-3-amine

ChemComp-8IX:
N-[(1r,3R,5S,7R)-3,5-dimethyltricyclo[3.3.1.1~3,7~]decane-1-carbonyl]-D-phenylalanine

Source
  • homo sapiens (human)
  • mus musculus (house mouse)
  • escherichia coli (E. coli)
KeywordsSIGNALING PROTEIN / GPCR

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