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TitleProteomic analysis of microtubule inner proteins (MIPs) in Rib72 null cells reveals functional MIPs.
Journal, issue, pagesMol Biol Cell, Vol. 32, Issue 21, Page br8, Year 2021
Publish dateNov 1, 2021
AuthorsAmy S Fabritius / Brian A Bayless / Sam Li / Daniel Stoddard / Westley Heydeck / Christopher C Ebmeier / Lauren Anderson / Tess Gunnels / Chidambaram Nachiappan / Justen B Whittall / William Old / David A Agard / Daniela Nicastro / Mark Winey /
PubMed AbstractThe core structure of motile cilia and flagella, the axoneme, is built from a stable population of doublet microtubules. This unique stability is brought about, at least in part, by a network of ...The core structure of motile cilia and flagella, the axoneme, is built from a stable population of doublet microtubules. This unique stability is brought about, at least in part, by a network of microtubule inner proteins (MIPs) that are bound to the luminal side of the microtubule walls. Rib72A and Rib72B were identified as MIPs in the motile cilia of the protist . Loss of these proteins leads to ciliary defects and loss of additional MIPs. We performed mass spectrometry coupled with proteomic analysis and bioinformatics to identify the MIPs lost in knockout axonemes. We identified a number of candidate MIPs and pursued one, Fap115, for functional characterization. We find that loss of Fap115 results in disrupted cell swimming and aberrant ciliary beating. Cryo-electron tomography reveals that Fap115 localizes to MIP6a in the A-tubule of the doublet microtubules. Overall, our results highlight the complex relationship between MIPs, ciliary structure, and ciliary function.
External linksMol Biol Cell / PubMed:34406789 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution17.3 Å
Structure data

EMDB-22712:
Subtomogram average of flagellar axoneme doublet from the wild type Tetrahymena thermophila
Method: EM (subtomogram averaging) / Resolution: 17.3 Å

EMDB-22713:
Subtomogram average of flagellar axoneme doublet from the Tetrahymena thermophila Fap115 knockout mutant
Method: EM (subtomogram averaging) / Resolution: 17.3 Å

Source
  • Tetrahymena thermophila (eukaryote)

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